Zetasizer Helix

 DLS data: Small size change  Raman data: 2° and 3° structure change  Combined data: Partial unfolding event Specifications

DLS light source

He-Ne Laser 633nm, Max 10mW

Raman light source

Diode Laser 785nm, Max 280mW at the sample

Dimensions (W, D, H)

320 x 600 x 375mm (Zetasizer) 580 x 450 x 200mm (Raman)

Weight

19Kg (Zetasizer) 28Kg (Raman)

150cm -1 to 1850cm -1 6cm -1 resolution

Spectral performance

Backbone - amount of α -helix, β -sheet, and random coil Tyrosine - hydrogen bonding extent, hydrophilic or hydrophobic environment Tryptophan – indole ring dihedral angle to backbone, extent of cation π interaction, if indole ring is buried or exposed, hydrophilic or hydrophobic environment Disulfide – Differentiate conformers, loss of covalent bond

Protein secondary and tertiary structural markers

Data collection modes

Isothermal incubation, Temperature ramp, Temperature jump, Sample / formulation series

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Zetasizer Helix brochure - MRK2127-02